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BurnAware Professional Premium 11.2 Crack [Extra Quality]



 
 
 
 
 
 
 

BurnAware Professional Premium 11.2 Crack

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A dominant selectable mutant of U937 cells was obtained by transfection of U937 cells with a pSV2 neo-derived plasmid carrying a frameshift mutant of the hypoxanthine guanine phosphoribosyltransferase gene. This dominant mutant was derived by selection with 6-thioguanine in the presence of the non-toxic concentration of butyric acid (Bt). Analysis of the mutant revealed that the mutant consisted of cells with one copy of the plasmid integrated into one of the chromosome 9 homologues. Cloning of the mutant was performed by transfecting the mutant cells with the plasmid carrying the HPRT gene. The presence of a second copy of the hypoxanthine guanine phosphoribosyltransferase gene restored HPRT activity and, as a consequence, sensitivity to 6-thioguanine in the mutant. Molecular analysis of the mutant revealed that the mutant represented a 30 base pair deletion in a region of HPRT coding for a sequence of 11 amino acids, corresponding to an alanine-aspartate motif.

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